HPLC method for residual DNA quantitation
Posted: Thu Feb 10, 2005 7:56 pm
Hi everyone,
We want to develop a LC method to quantify residual DNA in a biological extract composed in majority of proteins and lipids for using in a pharmaceutical purpose.
The major concern is that the fragments to be analyzed are of different size.
Ideally it would be nice to find a method to elute DNA in a single peak, regardless of the base pair number, but we surely will have to hydrolyze it in order to analyze single base pairs.
Does someone have experience with that kind of method? It would be very helpful
Thanks in advance
Martin
We want to develop a LC method to quantify residual DNA in a biological extract composed in majority of proteins and lipids for using in a pharmaceutical purpose.
The major concern is that the fragments to be analyzed are of different size.
Ideally it would be nice to find a method to elute DNA in a single peak, regardless of the base pair number, but we surely will have to hydrolyze it in order to analyze single base pairs.
Does someone have experience with that kind of method? It would be very helpful
Thanks in advance
Martin