By Anonymous on Tuesday, June 15, 2004 - 04:55 am:

hello everybody
we are doing amino acid analysis by HPLC after OPA/Fmoc derivatization (similar to Aminoquant)
do you know of a nice method to end up the system during week-ends or after a day sequence ?
thanks

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By Anonymous on Wednesday, June 16, 2004 - 01:24 am:

Do you mean to stop the machine?

I used to create a programe of finish with zero mL/min flow. Then, you programe another secuence with no sample.

If it's not what you ask, please tell me.

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By Anonymous on Tuesday, June 22, 2004 - 12:53 am:

not to stop the machine or the pump, the Agilent software has a function to do this
I am looking for a method to rinse the column and store it
thanks

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By RH on Friday, June 25, 2004 - 01:39 pm:

You should wash out the buffer salts by flushing
the column with a mixture of your organic
modifier and pure water (e.g. 50:50) with at
least 10 column volumes. Then you can run the
system with very low flow (0,02 ml/min) during
weekend.