Chromatography Forum

This is probably a simple question, but I'm a bit confused as to which is the right answer when using Chemstation.

Should the amount that I enter in the dilution field (in the sequence table) be just the dilution amount or should it be the sample amount + the dilution amount (total weight).

The version of Empower that I used to work with called for the "dilution" field to really mean the "total weight field" (sample + dilution).

An example in case I have worded this poorly would be say I had sample weight of 0.5g and then I diluted it in 10.0g of extraction solvent. Should the dilution field say 10.0g or 10.5g?

Thanks in advance.

This is probably a simple question, but I'm a bit confused as to which is the right answer when using Chemstation.

Should the amount that I enter in the dilution field (in the sequence table) be just the dilution amount or should it be the sample amount + the dilution amount (total weight).

The version of Empower that I used to work with called for the "dilution" field to really mean the "total weight field" (sample + dilution).

An example in case I have worded this poorly would be say I had sample weight of 0.5g and then I diluted it in 10.0g of extraction solvent. Should the dilution field say 10.0g or 10.5g?

Thanks in advance.

I thought dilution factor has to be entered into this field.
As an example: if your sample is dissolved in 0,5 mL of solvent and you dilute it to 1.5 mL by adding 1 mL of solvent then dilution factor is 3.

An example in case I have worded this poorly would be say I had sample weight of 0.5g and then I diluted it in 10.0g of extraction solvent. Should the dilution field say 10.0g or 10.5g?

Thanks in advance. :oops:

OK, if you're doing weight/weight:
Enter 0.5 in Sample amount.
Enter 10.5 g in Dilution.
If you would then dilute say 5 parts to a total of 100, then enter 20 into the "Multiplier" field.

I think the proper answer to this question depends on how you run your injections and what units you put on your calibrants. If your calibrants are concention units (i.e., ng/mL), and you always inject the same volume, in the example you gave of 0.5 g to 10.5 mL (assuming the sample is mostly water, like blood or even tissue, and extractrant does not have a negative volume of addition--hmm, lots of assumptions there...maybe you should measure the actual final volume), I would call the "dilution" 21.