PROTEIN STRUCTURE DETERMINATION
Posted: Tue Nov 09, 2010 10:30 pm
Is it possible to determine the structure of a unknown protein using Electron spray ionisation or it is possible only with MALDI
Primary structure refers to the sequence. Prior to MS you have to digest your protein. To analyze peptides after digestion you can use both ESI or MALDI but for small peptides ESI or matrix-free MALDI would be better.
Secondary structure: CD, FTIR
Tertiary and quaternary structure: fluorescence spectroscopy, NMR, X-ray