Chromatography Forum

I am trying to read up about Thermo LTQ/ LTQ-Orbitrap after seeing it in a job ad.

Anyone familiar with LTQ Orbitrap? What is Orbitrapâ„¢ technology?

What does ETD, HCD, CIS stand for? I know they are detectors:)

See
http://www.ionsource.com/Instrumentatio ... biTrap.htm

or
"
ETD Upgrades are available for LTQ Orbitrap and LTQ Orbitrap XL.

The Thermo Scientific LTQ Orbitrap XL ETD is the most powerful instrument for proteomics analyses: It combines three different and complementary fragmentation techniques CID, HCD, and ETD with the proven benefits of Orbitrapâ„¢ technology. It is the most comprehensive solution for complex PTM analysis, intelligent sequencing of peptides, top-down and middle-down analysis, and protein quantitation via stable isotope labelling such as iTRAQâ„¢ or label-free quantitation."

Wikipedia is your friend for Orbitrap.
ETD and HCD are two particular sorts of fragmentation available in addition to typical collision induced dissociation. ETD is a whizzy chemical approach that is recommended for delicate things (it's supposed to be able to break up peptides without knocking off phosphorylations, for example, so its useful for looking at protein modifications). HCD is a fragmentation not carried out in the LTQ's ion trap, but in an intermediate stage between the ion trap and the orbitrap part of the instrument. It's carried out in an environment that works much more like a collision cell, and as a result it doesn't suffer from the low-mass limit on fragment size that you see in virtually all ion trap instruments.
Good luck.

Wikipedia is your friend for Orbitrap.
ETD and HCD are two particular sorts of fragmentation available in addition to typical collision induced dissociation. ETD is a whizzy chemical approach that is recommended for delicate things (it's supposed to be able to break up peptides without knocking off phosphorylations, for example, so its useful for looking at protein modifications). HCD is a fragmentation not carried out in the LTQ's ion trap, but in an intermediate stage between the ion trap and the orbitrap part of the instrument. It's carried out in an environment that works much more like a collision cell, and as a result it doesn't suffer from the low-mass limit on fragment size that you see in virtually all ion trap instruments.
Good luck.

Thanks.