Urgent query
Posted: Wed Oct 27, 2010 10:35 am
I am trying to separate two molecules which differ only in that one has a hydroxyl group and the other a chloride in that position
Chromatography Forum
Chromatography Forum is a public discussion group where you can post questions, news, or messages of interest to chromatographers everywhere, but you must be registered to participate (registration is FREE). If you are a registered user, please log in.
http://www.chromforum.org/
Urgent query
Page 1 of 1
Posted: Wed Oct 27, 2010 11:37 am
good luck!
Posted: Wed Oct 27, 2010 8:00 pm
Details?
Posted: Thu Oct 28, 2010 2:44 am
There are many molecules with hydroxyl groups and chlorine atoms - and they take many kinds of separatoin techniques, based on the types of molecules they are.
Posted: Wed Nov 10, 2010 11:54 pm
Try a standard C18 column and a standard HILIC column first. It depends which group has more influence, charge etc.! But without more details on the compounds or what you tried so far it is not possible to give you more detailed information.
Posted: Fri Nov 12, 2010 2:10 pm
I would say that should be "standard fare" for a reveresed phase separation gradient or isocratic on a bog standard C18 column....
Posted: Tue Nov 16, 2010 5:16 am
That's nice! 