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Lysine
Posted: Fri Oct 08, 2010 6:01 am
by dude
Hi everyone!
Im trying to analyze lysine in an RP column(C18) in a PDA detector but still no peak in the elution. can you give me any suggestion? thnx everyone!
Posted: Fri Oct 08, 2010 9:15 am
by danko
I think you'll need to find another detection technique (not UV absorption based) or derivatize the lysine.
Best Regards
Posted: Fri Oct 08, 2010 12:22 pm
by Chromatographer2010
You could probably see something below 210 depending on if your mobile phase is transparent down there. i.e. really clean ACN and water with no additives. Sensitivity will still be extremely poor.
Lysine
Posted: Sat Oct 09, 2010 6:32 am
by brbabu_1979
Hi dude,
Lysine is an amino acid. Generally amino acids are not detected with UV. It requires derivatization. If you search on Google for amino acid derivitisation methods you can get some and may helps you.
Best regards
Babu
Posted: Sat Oct 09, 2010 6:45 am
by SIELC_Tech
you will not have too much retention on C18 column without IP reagent.
you can see lysine in UV at 200-215 nm. here is few applications including one with UV detection:
http://www.sielc.com/compound_057.html
contact me if you have questions
Posted: Mon Oct 11, 2010 3:18 am
by GaryR
Dude! Where's my Lysine?
Sorry, couldn't help myself!
Lysine, using UV/Vis detector
Posted: Mon Oct 11, 2010 9:36 am
by Khanom
Have you tried using pre-column derivatisation with O-Phthalaldehyde (OPA) in a borate buffer, giving UV absorbing iso-indoles. Run this on a Hypersil Gold column at 40 C, using a binary gradient . Use a binary gradient of 40mM NaH2PO4/Na2HPO4 (1:1) buffer pH=7.8 and AcN/MeOH/H2O (45/45/10 v/v/v). Detect at 338 nm. Lysine peak should elute at around 15 minutes.
Posted: Tue Oct 12, 2010 12:52 am
by dw237606