chitosan oligomers analysis

[thohry]

Hi all,

We have to analyze chitosan oligomers. The conditions in the application is :

Column: aminopropyl ( 300x3.9x5), with ACN/H2H 70:30. RI detection.

But we do not have aminopropyl column but Amirex (HPX-87C) from BioRad.
Can I use instead and if yes, what are the condittions?

Thanks for any help

[mardexis]

You can try the Aminex but I'm going to guess no way. Glucosamine comes out near the void and your larger oligomers are going to come out sooner so I would expect all oligomers of glucosamine to coelute.

The aminopropyl sounds promising.

[thohry]

You can try the Aminex but I'm going to guess no way. Glucosamine comes out near the void and your larger oligomers are going to come out sooner so I would expect all oligomers of glucosamine to coelute.

The aminopropyl sounds promising.

Thank you for the help. I will come back to the amiino column.