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new column

Posted: Tue Oct 05, 2010 1:40 pm
by ossa97
how should i condition this new column:: BDS HYPERSIL C18 5MICRON 250X4.6

Re: new column

Posted: Tue Oct 05, 2010 3:18 pm
by scientist
how should i condition this new column:: BDS HYPERSIL C18 5MICRON 250X4.6
In my view you should run pure Methanol for at least 30 minutes and the use the mobile phase!


Have a nice time

:D

Posted: Tue Oct 05, 2010 9:20 pm
by Uwe Neue
Why?

Posted: Wed Oct 06, 2010 7:38 am
by scientist
I have seen many columns COA where thay state that the column is shifted with Methanol inside it, whether Methanol or ACN is used as mobile phase.

I think u should see the Column COA, u will notice it as well.