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amino column : reverse or normal ?

http://www.chromforum.org/viewtopic.php?t=1397

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amino column : reverse or normal ?

Posted: Tue Feb 01, 2005 9:24 am
by fa
Hello,
I want to separate sugars with an amino column and a RI detector. The mobile phase should be CH3OH/H2O (75/25). As the mobile and the stationnary phase are polar, is it a normal or a reverse phase chromatography ?
Thank you

Posted: Tue Feb 01, 2005 2:52 pm
by tom jupille
If you increase the amount of ACN in the mobile phase, retention increases, therefore it is, technically, normal-phase. If this technology were developed today, it would be classed as HILIC (see other threads on that topic).
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