Chromatography Forum

Hi,

I am attempting to analyze a compound (via HPLC) that is in a product which contains gums (iota-carrageenan, xanthan, and locust bean gum). Does anyone have suggestions of a good solvent that I could use to break down/dissolve the gums so that I could extract the active?

I've done a bit of research and ran into chloroform and hexane as possibilities.

Thanks in advance.

this strongly depends on your compound of interest characteristics and method of separation

dissolving in chlorform hexane with no other steps means going for a normal phase method in HPLC most likely. is it suitable?

you could do a liquid to liquid extraction or SPE, again depending on the nature of your compound

The problem with many gums is that if you disslove them, they usually thicken up the solvent. That makes them hard to inject then. Is there a way to extract the compound from the sample, leaving the polysaccharides behind?