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How to analyse collagen content.

http://www.chromforum.org/viewtopic.php?t=13823

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How to analyse collagen content.

Posted: Sat Sep 11, 2010 4:11 am
by Nuunoo
Dear All,

I need to analyse collagen content in food,I don't have idea.
Please suggestion for me.
Thanks in advance for all your help.

Posted: Sat Sep 11, 2010 3:26 pm
by carls
Take a look at these references (full text).

They should get you started:

Full procedure including digestion and colorimetric determination:
http://www.scielo.br/scielo.php?script= ... 9000300023


Full procedure including digestion and HPLC analysis using post column derivatization:
http://www.ars.usda.gov/SP2UserFiles/Pl ... llagen.pdf

Posted: Sun Sep 12, 2010 10:06 am
by Nuunoo
Thank you very much.
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