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How to analyse collagen content.

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

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How to analyse collagen content.

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Nuunoo
Dear All,

I need to analyse collagen content in food,I don't have idea.
Please suggestion for me.
Thanks in advance for all your help.

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carls
Take a look at these references (full text).

They should get you started:

Full procedure including digestion and colorimetric determination:
http://www.scielo.br/scielo.php?script= ... 9000300023


Full procedure including digestion and HPLC analysis using post column derivatization:
http://www.ars.usda.gov/SP2UserFiles/Pl ... llagen.pdf
A. Carl Sanchez

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Nuunoo
Thank you very much.
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