Chromatography Forum

Hi all,

I am working on a enzyme assay. One of products is dihydroxyacetone.
I 've tried to use LC-MS/MS to measure it ( positive mode MRM: 91 to 61, cone 15, CE 7), but it had poor LOD (S/N =3) at around 250 uM. I wonder if it is possible to optimize sensitivity and measure it by LC-MS/MS. Or GC-MS can work better for it (GC-MS is not available right now)?

Thanks!

Did you try HILIC or RP as your LC method? In RP, it will be unretained and you my have a ton of interferences. Also, HILIC methods give you a higher sensitivity in MS detection.

For such a low molecular weight compound, your better bet is GC-MS, maybe with some derivatization.