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how to understand pore size, surface area and carbon load?

Posted: Fri Sep 03, 2010 11:33 am
by guoyang
Hi, everyone!
Could you tell me how to understand pore size, surface area and carbon load of a HPLC column and their effection to chromatographic peak? For example: retention, peak shape and so on.
Thanks!

Posted: Fri Sep 03, 2010 3:16 pm
by tom jupille
Entire books have been written to that end. I would recommend getting a copy of Uwe Neue's HPLC Columns: Theory, Technology, and Practice. Here's a link to the information on Amazon:
http://tinyurl.com/2ddyx8h

Posted: Sat Sep 04, 2010 10:22 am
by guoyang
Thank you very much!