buffer selection mobile phase

[gaucholeonardo]

Hi

I'm using phosphate buffer 0,05M and ACN (85:15) but the time retention is about 3 min, the pKa of drug that i'm analyzing is about 8... someone can help me?
how can i select the correct mobile phase? and how the sample prepare can cause interference in the analysis?
anyone know a book/article to indicate?

tks

Leo

[rhaefe]

A good start would be IMHO:

<1> Practical HPLC Method Development by Snyder, Kirkland, Glaijch
<2> Pitfalls and Errors of HPLC in Pictures by Veronika R. Meyer
<3> Practical High-Performance Liquid Chromatography by Veronik R. Meyer
<4> The HPLC Solvent Guide by Paul Sadek


cheers,

rh