3 peaks of bromazepam?
Posted: Thu Aug 26, 2010 2:41 pm
Good afternoon,
I have a problem with Bromazepam in my HPLC. When I make the chromatograph for it I have 3 peaks. The first two peaks are together and with one line in a high level, I mean, it seems an equilibria reaction. I'm using a C18 column 12x0.46,3µm. The solvent is 50%MeOH+50%ACN and the aquous phase is formed by NaH2PO4 + H3PO4 (2.6pH).
Could you explain me the reason, please?
Thank you!
C.Romero
I have a problem with Bromazepam in my HPLC. When I make the chromatograph for it I have 3 peaks. The first two peaks are together and with one line in a high level, I mean, it seems an equilibria reaction. I'm using a C18 column 12x0.46,3µm. The solvent is 50%MeOH+50%ACN and the aquous phase is formed by NaH2PO4 + H3PO4 (2.6pH).
Could you explain me the reason, please?
Thank you!
C.Romero