Calculating on-column amounts for purge and trap

[tangaloomaflyer]

How I do calcualte the on-column amount of analyte for purge and trap GCMS?

Presumably I need to know the purge and desorb efficiency?

thanks

[Peter Apps]

This can rapidly become a circular argument - on-column amounts are calculated on the basis of sorbtion and desorption efficiencies, and split ratios, and efficiences are calculated as on-column amounts vs standards added to traps

You can calibrate peak area vs on-column amount using splitless or preferably onc olumn injections of standards in solvent, then the peak area from the P&T runs gives you the on-column amount.

Peter

[AICMM]

tangaloomaflyer,

Re: purge and desorb efficiency, for most compounds (the ones that purge well) I would argue no, you don't. But if you want I would propose the following. Purge a standard and run it. Don't allow your trap to bake. Re-run the trap on the next run. Look at the area recovered on a subsequent desorb. Then re-IS your standard water with just a bit of fresh IS in water and re-purge and re-run the same water.

Best regards,

AICMM