Advertisement

HPLC detector

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

3 posts Page 1 of 1
Dear
Why we are using PDA for analytical development?
well, it's very important for us to decide the determine wavelength.Because by uv we can't see all constituents, such as related substances. By PDA we can choose a better wavelength which can determine all constituents. I think PDA is helpful for you to develope an method.

In addition to what bingyechumen has said, you can also extract spectra from peaks in your chromatogram. Often in method development we will see peaks co-elute, and PDA will help us in "telling" the purity of the peak (given that the spetra for co-eluting analytes are sufficiently different).
3 posts Page 1 of 1

Who is online

In total there are 328 users online :: 0 registered, 0 hidden and 328 guests (based on users active over the past 5 minutes)
Most users ever online was 10230 on Thu Dec 04, 2025 5:56 am

Users browsing this forum: No registered users and 328 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry