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Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
Excuse me ,you have said that the compound is" very basic",however ,it has a -COOH??Tazobactam is very basic compound. Please let us know which C18 column you are using, and why you have choosen pH3. Increase in retention time can be linked to the fact that more and more active silanol groups are on the surface.
Maybe a more basic pH and or a very well base deactivated column would give more stable retention times.
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