Chromatography Forum

hi

i am anew friend in this php .if any one can help me to fined the suitable hplc method for acarbose .i havenot NH2 column if there is another column i can use it instead of NH2 column

Don't bother with a NH2 column. You only will have trouble.

Look for the BEH Amide column at the Waters website.

I also would say that NH2 column is not the best choice in terms of reproducibility.
If you google for Acarbose you will find a lot of published methods. From Anion exchanger, to special sugar columns, affinity columns, base deactivated RP-18 columns, standard RP-18 columns to NH2 columns. My recommendation is to use a well endcapped RP-18 of the new generation columns. I would go for a so called polymeric bonded column, means trifunctional bonded, or difuncional bonded with a difunctional bonded endcapping, where you can use up to 0% organic modifier in mobile phase, or just try HILIC. Please take into consideration to use a volatile buffer, if you want to use MS detection later on. Anyhow, if you have a virgin RP-18 column in your lab, endcapped, you can use it. Good luck

you could try a carboPac PA1 dionex column for IC they have many applications for sugars
or a Sugar-D cosmsil column with 70% ACN/30% water

You can analyze this compound using HILIC column. Here is application for other sugars. In case of higher molecular weight sugars you need to use less ACN (60-65% for your molecule):
http://www.sielc.com/pdf/Sugars%20on%20 ... column.pdf

Below is separation for voglibose and acabarose on Unison UK-Amino:

http://www.imtaktusa.com/site_media/fil ... TI402E.pdf

Hello membres,
I communicate to you the analytical method of Acarbose dosing in a finished product.
The chromatographic conditions for the determination of AI in the FP are the same as in the monograph PH EUR for this AI. However, applying this method in our laboratories, we find a very unstable TR: Tr said 16 minutes, after some injections he shifts and even gets to 9 minutes (! Sometimes less). the column used is: Hypersil APS-2 250 mm × 4 mm, 120 am-5. µm.
Thank you tell us if he has any special precautions for this column.

The APS-2 column is an amino bonded-phase column. If you read the earlier posts in the thread, you will see that experts (including the late and much-missed Uwe Neue) do not recommend that type of column. I would suggest you check with Thermo to see if they can suggest a specific alternative.