ion pairing amines
Posted: Thu Aug 12, 2010 4:15 pm
by mardexis
I want to run oligonucleotides by LC/MS. Currently use HPLC with ion pairing reagents... either hexylamine acetate or triethylamine acetate. Does anyone have experience running these ion pairing reagents into their LC/MS?
Posted: Tue Aug 17, 2010 8:21 am
by rhaefe
Christian Huber et al. did a lot of work on nucleic acids and LC-MS. One example here:
http://pubs.acs.org/doi/abs/10.1021/ac990378j
Triethylammonium bicarbonate is also a useful ion-pairing reagent for your prurpose. It is more volatile than TEAA. The pH is higher about 8 to 8.3. Fluka (part of Sigma Aldrich) used to sell a 1.0mol/L stock solution. I used it quite successfully to separate dsDNA. But not in LC-MS mode.