Ibandronate sodium

[marandrade]

Hi, I´m looking method analysis HPLC for Ibandronate sodium, someone help me?

[tom jupille]

Try Google on the keywords "Ibandronate sodium" and HPLC

[Vlad Orlovsky]

Here is a method for "dronic" acids:
http://www.sielc.com/compound_249.html

Also here is reference on validated method on similar compound (etidronic acid):

Direct stability-indicating method development and validation for analysis of etidronate disodium using a mixed-mode column and charged aerosol detector. Liu, X.K., Fang, J.B., Cauchon, N., Zhou, P. Journal of Pharmaceutical and Biomedical Analysis , Vol. 46, No. 4, pages 639-644 (2008)

Contact me if you have questions.

[Mohammad Elshahawy]

This material really drove me crazy.
if you want to develope a method without a high sensitivity, you can use Refractive index detector.
we have only UV and Flourscence detectors in our lab, that's why I tried to derivatize the compound with a flourogenic agent Fmoc, but the Amino group was very hard to derivatize, and the results were so poor.

I tried to undergo estrification of the hydroxyl group, with phthalic anhydride, which is a good chromogenic agent, but I needed very high concentration of the phthalic anhydride to push the reaction to completion and the remaining phthalic acid peak dwarfed the ibandronate phthalate peak and cause poor separation.

I tried to use some catalyst "pyridine" although all my colleagues were so angry about it but even it didn't make a good job.

well, I told my boss to buy a refractive index detector for our HPLC as it was a big failure for me, and still it hurts my feelings LOOOL.

A friend of mine has a refractive index detector at his lab, and he could develop a method for it.

[shyam]

try C18 column with
50mm Sodium acetate and 50mm KH2PO4 with 0.5g hexane sulphonate with ph 3.5 with acetic acid and acetonitrile.