Chromatography Forum

Hi ,

If the police bring to you a material that is unknow , from where to start to say this materials contains ( x, y , z , n ) harmfull materials .In case if you have RP-HPLC is it possible to know by comparing retention time ( of course not because we know that some cmpds have the same Rt ). I know many will use GC-MS OR LC-MS , BUT do you thing this will help to identify the compounds exactly , does this want specific method but how you can know the method and this material is completly unknown . is the library that is attached is trustabe ? . if you say it is 1 ppm other instrument will say 20 ppm !

By the way , are there books that we can follow to find what harmfull cmpds that are in mint , or vegetables , fruits etc .

I know the questions are a bit difficult so don't forget to say hello by answering me .

Thanks

WESAM, I was nice to you last time, but you *are* stretching things a bit.

The deal on exam questions is this: you answer as best you can, and we'll either validate your answers or provide hints to point you in the right direction.

It is not an exam question believe me Tom , i just want to ask about the culture a round this area i swear just for knowledge not for exam ! .I was expected someone to say ' buy the EPA method on HPLC it will tell you about every things in regualted methods '. or see this web as an example .

Because my friend open in his country small lab to deal with testing mint , vegetables and he ask me to find method for him . The scientific papers are really ambigous we can not understand from it .So i said maybe someone will help to find out about this .

Any way , Thanks for you

Think about where the sample comes from, think about what it "might" contain, think about waht sorts of things you *should* be looking for.

Samples of vegetables - you might be interested in pesticides, airborne contaminants and things in the groundwater.

Meat samples - you're probably going to be interested in hormones, antibiotic metabolites, e-coli and prions.

The more specific your list of things you want to test for, the easier it will be to find methods.