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retention time related guard column

http://www.chromforum.org/viewtopic.php?t=1358

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retention time related guard column

Posted: Mon Jan 24, 2005 4:28 pm
by irene_wu
hi, guys:
thank you all for helping me to solve the problem about back pressure. now I have a question about the retention time:

the retention time for same solution on column directly and column with guard column are same. why?suppose there should be some differences due to the total column lenth .

welcome any explaination :?:

Posted: Mon Jan 24, 2005 7:08 pm
by tom jupille
Actually, it should be longer by the increase in column volume. If you have a small guard cartridge, the differnce may only be a couple of percent.
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