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High Throughput Sample Prep

Discussions about sample preparation: extraction, cleanup, derivatization, etc.

6 posts Page 1 of 1
Hi, all -

I am interested in increasing the throughput of my lab by improving my sample prep process.

My lab sees about 3000 to 3500 samples a month, and we run 3 to 5 HPLCs (depending on which methods we're running).

Our current process is to receive samples in 1.5 mL microcentrifuge tubes, spin each one, pull off supernatant and filter through a syringe filter into 1 mL HPLC vials.

I have systems (Shimadzu) that could be adapted to 96 well plate sampling. I hear they've also come out with a sample tray changer, which could be helpful.

Does anyone have any good suggestions for centrifuging and filtering 100 - 150 samples per day, and loading onto the HPLC?

I'm in the information gathering stage now. I'm sure there are good ideas out there to help me.

Thanks for any input.

H_H

http://www.whatman.com/SyringelessFilters.aspx

From Whatman, could eliminate the centrifugation step.

They're a bit expensive though
Good judgment comes from bad experience, and a lot of that comes from bad judgment.

Not enirely clear what you are trying to do. You may want to look at the following option (Sirocco 96 well plates): http://www.waters.com/webassets/cms/lib ... a31784.pdf

More sample preparation:
http://www.gelifesciences.com/aptrix/up ... reparation

Best Regards
Learn Innovate and Share

Dancho Dikov

Thank you, all. I'll check these out.

Uwe, what I am trying to do is eliminate having to individually centrifuge and filter 100 samples a day, and instead maybe use multi channel pipettes to load well plates, or something to that effect. Or maybe there are plates that can be centrifuged. I am not exactly sure what I need!

Our samples are very dirty, and so both centrifugation and filtration appear to be necessary.

Thanks again, everyone.

H_H

MicroLiter has ITSP....

ITSP (Instrument Top Sample Prep) is a consumable device for solid phase extraction, or for filtration, designed to enable a 3-axis autoloader to automatically prepare samples on a standard analytical instrument workstation.
Who should consider using ITSP?
Analytical chemistry laboratories in academic institutions, government, and industry operating in the following fields:
1) Pharmaceutical (for drug discovery and development)
2) Forensics/Toxicology (for analyzing physical evidence)
3) Clinical (for analyzing patient samples)
4) Environmental (for analyzing field samples)
5) Industrial (for QA/QC)
a. Food & Beverage
b. Petrochemical
c. Cosmetics
d. Consumer Goods
6) Contract Laboratory (for any of the above)
When should a lab consider using ITSP?
When, as part of your sample analysis, you commonly:
1) Selectively extract a compound of interest from a sample matrix using Solid Phase Extraction, or
2) Concentrate a sample using Solid Phase Extraction, or
3) Remove particulates from a sample using Filtration, or
4) Remove inorganic salts from a sample by passing through a nonpolar sorbent.
Where should a lab consider using ITSP?
On an analytical instrument workstation (e.g. LC, GC, MS) having a CTC Analytics or LEAP PAL (or similar 3-axis autoloader), e.g., CTC GC PAL CTC HTC PAL CTC HTS PAL CTC HTX PAL CTC IFC PAL Prep CTC IFC PAL Analytical CTC Combi PAL CTC Combi Headspace PAL
ITSP – 5 W’s Page 2
MicroLiter Analytical Supplies, Inc.
Why should a lab consider using ITSP?
1) To reduce capital equipment costs
2) To reduce cost of consumables
3) To reduce cost of solvents
4) To reduce cost of solvent disposal
5) To increase quality of results
6) To reduce labor costs
7) To increase lab production
8) To reduce risk of cross-over
9) To reduce errors
10) To improve chain-of-custody verification
How does ITSP provide the benefits listed above?
1. Since ITSP is designed to operate on (often) pre-existing, relatively inexpensive, 3-axis autosamplers in the workstation, it eliminates the requirement to acquire more expensive equipment in order to automate sample preparation.
2. ITSP reduces the cost of consumables by:
a. Reducing the quantity of glass vials , inserts, caps, and micro-plates needed to perform sample preparation, and
b. Requiring less sorbent material per sample by facilitating the scaling down of method volumes.
3. ITSP reduces the cost of solvents by facilitating the scaling down of method volumes.
4. ITSP reduces the cost of solvent disposal by facilitating the scaling down of method volumes.
5. ITSP increases the quality of lab results since it enables the automation of sample preparation methods, which reduces sample-to-sample variation compared to manual processing.
6. ITSP reduces labor costs by facilitating the automation of sample preparation, which:
a. Allows the autoloader to perform many of the tasks formerly performed by lab technicians, freeing up their time for other tasks, and
b. Allows for delegation of tasks to less skilled, and therefore less costly, technicians.
7. ITSP increases lab production by enabling the autosampler to prepare the next sample while the previous sample is being analyzed by the analytical instrument(s), thereby reducing dead-time of the autoloader.
8. Since the ITSP device is a single-use consumable, the opportunity for cross-over between samples is reduced, compared to applications utilizing re-usable devices.
9. ITSP reduces errors since an inherent benefit of automating any process is the reduction of potential for human error.
10. ITSP improves the verification of chain-of-custody in evidentiary sample processing because, once a method is properly automated and validated, the sample preparation and handling can be documented through automated performance re
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