Advertisement

Agilent system airlocked

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

3 posts Page 1 of 1
left it pumping over the weekend, 0.2ml/min, 50/50

came in yesterday and system full of air, not sure how as there was enough solvent
how can I fix this?

TIA

What is the solvent you are using? If this happens to me I ususally prime the system again and then push isopropanol through (higher viscosity - helps to push air through).

cheers, I tried methanol and that seemed to work

one thing I notice was that the filter was releasing air bubbles, took this off then no more air
3 posts Page 1 of 1

Who is online

In total there are 63 users online :: 2 registered, 0 hidden and 61 guests (based on users active over the past 5 minutes)
Most users ever online was 4374 on Fri Oct 03, 2025 12:41 am

Users browsing this forum: Ahrefs [Bot], Semrush [Bot] and 61 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry