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TFA vs. Formic Acid
Posted: Fri Jul 23, 2010 3:51 pm
by Yuhuan Tang
We sometime have unwanted blank peak when using formic acid in mobile phase, I was told that TFA is a better replacement for formic acid. Beside possible suppression of the MS signal do you have any other issues when using TFA?
Please share your experience.
Thanks,
yt
Posted: Fri Jul 23, 2010 7:28 pm
by usuchrom
Will you use formic acid at 99% pure? I use this and I have no "ghost" peaks. About the TFA, I think that it do not harm the MS signal. The only thing against the TFA is that during chromatography could Trifluoroacetyl some products.
Posted: Mon Jul 26, 2010 2:41 pm
by alexdwsn12
TFA:
-Trifluoroacetic acid (TFA) is the simplest stable[1] perfluorinated carboxylic acid chemical compound, with the formula CF3CO2H.
- The use of trifluoroacetic acid in API-Electrospray may lead to the formation of ion pair with analytes and the consequence is ion suppression.
Formic Acid :
- Formic acid have a good volatility and is capable of lowering pH at value 2-3: therefore it is good choice in API-ES for compounds with a protonable group.