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Neomycin Sulfate Method
Posted: Thu Jan 20, 2005 11:54 pm
by Sallybeetle
I am trying to find a HPLC Assay / Stability Indicating method for Neomycin Sulfate. Anybody know of a method or where to look? Web search didn't turn up anything.
Posted: Fri Jan 21, 2005 12:12 am
by Uwe Neue
Posted: Fri Jan 21, 2005 12:05 pm
by Ann
Adams E, Schepers R, Roets E, Hoogmartens J. Determination of neomycin sulfate by liquid chromatography with pulsed electrochemical detection. J Chromatogr A. 1996 Aug 16;741(2):233-40.
(Try a PubMed search, several more published HPLC methods revealed when searching for 'neomycin sulphate HPLC'. Some are methods for quantifying other aminoglycosides but have used neomycin as an internal standard).
Best wishes
Ann
Posted: Fri Jan 21, 2005 1:00 pm
by SIELC_Tech
Here is the reference for gentamicin sulfate. It is isocratic and does not have hexafluoro butiric acid (ion-pairing reagent) as additive. Your compound will retain slightly less because of two amino sugar rings vs. three.
Method is compatible to preparative chromatography (ACN-water-TFA)
http://allsep.com/makeCmp.php?cmp=Cmp_142
Posted: Fri Jan 21, 2005 3:47 pm
by AA
Chromatographic methods for analysis of aminoglycoside antibiotics
Isoherranen, N.; Soback, S.
Assoc. Off. Anal. Chem. Int.
1999, 82(5) Page: 1017
Posted: Fri Jan 21, 2005 5:05 pm
by Patrik Appelblad
Check out the following papers by Oertel et al
Determination of neomycin by LC-tandem mass spectrometry using hydrophilic interaction chromatography.
J Pharm Biomed Anal. 2004 May 28;35(3):633-8.
and by the same group
Hydrophilic interaction chromatography combined with tandem-mass spectrometry to determine six aminoglycosides in serum.
J Chromatogr A. 2004 Nov 26;1058(1-2):197-201.
Good Luck,
Patrik