-
- Posts: 13
- Joined: Mon Nov 24, 2014 3:17 pm
I am doing research on phenolic compounds in alcoholic beverages. Could someone working with compounds like caffeic acid, epicatechin, catechin, chlorogenic acid, rutin, quercetin, procyanidins, etc. please indicate what LOD one could expect from a triple quadrupole instrument in fullscan mode for these compounds.
Perhaps what would also be helpful is another LOD for any other compound on a triple quadrupole instrument, because I will be able to use this as a benchmark for our own instrument. We are struggling to see anything below 5 mg/L in fullscan and we know for sure there is some form of metal ion contamination that has influenced our data. So, this scanning ability is definitely NOT normal.
In case you would like some more detail about the analytical method:LC conditions were as follows: 0.1% acetic acid in water in A and LC-MS grade acetonitrile in B. Starting conditions 5% B at a flow of 0.3 ml/min through a Acquity UPLC BEH C18 (1,7 micrometer, 2.1x100 mm) column increasing to 90%B during 17 minutes with a 3 minute return to starting conditions. MS conditions were as follows: Capillary at 2 kV, cone at 30 V, desolvation temp. at 600 degrees C, desolvation gas flow at 700 L/hour, cone gas flow at 50 L/hour and source temp. of 130 degrees C. Waters Xevo TQD mass spectrometer. Acquity I-class.
Perhaps what would also be helpful is another LOD for any other compound on a triple quadrupole instrument, because I will be able to use this as a benchmark for our own instrument. We are struggling to see anything below 5 mg/L in fullscan and we know for sure there is some form of metal ion contamination that has influenced our data. So, this scanning ability is definitely NOT normal.
In case you would like some more detail about the analytical method:LC conditions were as follows: 0.1% acetic acid in water in A and LC-MS grade acetonitrile in B. Starting conditions 5% B at a flow of 0.3 ml/min through a Acquity UPLC BEH C18 (1,7 micrometer, 2.1x100 mm) column increasing to 90%B during 17 minutes with a 3 minute return to starting conditions. MS conditions were as follows: Capillary at 2 kV, cone at 30 V, desolvation temp. at 600 degrees C, desolvation gas flow at 700 L/hour, cone gas flow at 50 L/hour and source temp. of 130 degrees C. Waters Xevo TQD mass spectrometer. Acquity I-class.
