Caffeine in Positive ESI Mode
Posted: Tue Jul 20, 2010 2:32 pm
by ladybug
Hello, does anybody have a good mobile phase composition and gradient recomendation to run Pure Caffeine Standard (1mg/ml) on an LCMS in positive ESI mode? Would ACN:H2O do the trick? Column is C18. Thank you in advance!
Posted: Thu Jul 22, 2010 6:46 am
by Alp
Are you sure you are injecting samples in the mg/ml range or do you mean mcg/ml?
A quick google turned up...
http://www.shimadzu.com.br/analitica/ap ... cms016.pdf
among others.
Alp
Posted: Thu Jul 22, 2010 3:04 pm
by ladybug
Sorry it's in ug/ml not mg/ml.
Thank you alp!