Neomycin Sulfate Method

[Sallybeetle]

I am trying to find a HPLC Assay / Stability Indicating method for Neomycin Sulfate. Anybody know of a method or where to look? Web search didn't turn up anything.

[Uwe Neue]

Here is one:

http://www.waters.com/watersdivision/pd ... ntis65.pdf

[Ann]

Adams E, Schepers R, Roets E, Hoogmartens J. Determination of neomycin sulfate by liquid chromatography with pulsed electrochemical detection. J Chromatogr A. 1996 Aug 16;741(2):233-40.

(Try a PubMed search, several more published HPLC methods revealed when searching for 'neomycin sulphate HPLC'. Some are methods for quantifying other aminoglycosides but have used neomycin as an internal standard).

Best wishes

Ann

[SIELC_Tech]

Here is the reference for gentamicin sulfate. It is isocratic and does not have hexafluoro butiric acid (ion-pairing reagent) as additive. Your compound will retain slightly less because of two amino sugar rings vs. three.
Method is compatible to preparative chromatography (ACN-water-TFA)

http://allsep.com/makeCmp.php?cmp=Cmp_142

[AA]

Chromatographic methods for analysis of aminoglycoside antibiotics

Isoherranen, N.; Soback, S.
Assoc. Off. Anal. Chem. Int.
1999, 82(5) Page: 1017

[Patrik Appelblad]

Check out the following papers by Oertel et al

Determination of neomycin by LC-tandem mass spectrometry using hydrophilic interaction chromatography.

J Pharm Biomed Anal. 2004 May 28;35(3):633-8.

and by the same group

Hydrophilic interaction chromatography combined with tandem-mass spectrometry to determine six aminoglycosides in serum.

J Chromatogr A. 2004 Nov 26;1058(1-2):197-201.


Good Luck,

Patrik