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FAO Uwe de Neue: Proven ion-pair method for choline?

Posted: Tue Jul 20, 2010 10:37 am
by chromeleon
I need to do this by indirect UV detection. can anyone help?

T.I.A.

Posted: Thu Jul 22, 2010 4:38 pm
by chromeleon
where have all the experts gone??

Posted: Thu Jul 22, 2010 8:53 pm
by bisnettrj2
J Pharm Biomed Anal. 1995 Apr;13(4-5):581-8.

Ion-exchange liquid chromatography method with indirect UV detection for the assay of choline in pharmaceutical preparations.
Leroy P, Barbaras M, Colin JL, Nicolas A.

Laboratoire de Chimie Analytique, URA CNRS 597, Faculté des Sciences Pharmaceutiques et Biologiques-B.P. 403, Nancy, France.


I'm no expert, I'm just friends with Google.

Posted: Mon Jul 26, 2010 12:14 pm
by chromeleon
thank you, but that was one of the first papers I found :lol:
it is useful but not exactly what I need

on a related note, how can I reverse the polarity of my UV detector?

Posted: Fri Jul 30, 2010 1:51 pm
by chromeleon
baseline is all over the place
apart from filtering my buffer and controlling column temperature, any other tricks?