Advertisement
Chromatography Forum

Caffeine in Positive ESI Mode

Discussions about GC-MS, LC-MS, LC-FTIR, and other "coupled" analytical techniques.

3 posts Page 1 of 1
Post a reply

Caffeine in Positive ESI Mode

avatar
ladybug
Hello, does anybody have a good mobile phase composition and gradient recomendation to run Pure Caffeine Standard (1mg/ml) on an LCMS in positive ESI mode? Would ACN:H2O do the trick? Column is C18. Thank you in advance! :)

avatar
Alp
Are you sure you are injecting samples in the mg/ml range or do you mean mcg/ml?

A quick google turned up...

http://www.shimadzu.com.br/analitica/ap ... cms016.pdf

among others.

Alp

avatar
ladybug
Sorry it's in ug/ml not mg/ml.

Thank you alp!
Post a reply
3 posts Page 1 of 1
Return to “LC-MS, GC-MS, and other”

Who is online

In total there are 19 users online :: 0 registered, 0 hidden and 19 guests (based on users active over the past 5 minutes)
Most users ever online was 4374 on Fri Oct 03, 2025 12:41 am
Users browsing this forum: No registered users and 19 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

    Gas Chromatography

      Mass Spectrometry