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GPC problem, help

http://www.chromforum.org/viewtopic.php?t=13351

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GPC problem, help

Posted: Thu Jul 08, 2010 12:45 pm
by Dori
I have a Styragel HR1 Column (THF). Methanol was used as eluyent by mistake for about 3 hours. Now I don't obtain good resolutions, probably the column is damaged.
Is it possible to fix it? Thanks.
Dory

Posted: Thu Jul 08, 2010 1:18 pm
by Uwe Neue
If it does not perform any more after being converted back to THF, it is probably dead. Polymeric packings swell and shrink significantly as a function of the solvent. THF is a swelling solvent, methanol will shrink the packing.
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