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n00b looking for iron (Fe) on HPLC
Posted: Tue Jul 06, 2010 2:57 pm
by LFH
Hi, I am trying to use an HPLC to determine the presence of two metals- Fe and Pd. We have developed a Pd method using a chelating agent NaDEDTC that shows up at 300 nm. Currently we do not have a recipe for Fe detection, but I am surmising that there should be an easy way using EDTA? Does the complexed and uncomplexed EDTA show up differently? can I use that fact to determine how much iron is taken out of solution (assuming the solution is at equilibrium)?
any suggestions would be much appreciated!
Posted: Tue Jul 06, 2010 4:22 pm
by tom jupille
Some ideas in this thread:
viewtopic.php?p=12368
Still need help!
Posted: Fri Jul 09, 2010 12:57 pm
by LFH
Sorry- it seems like many have read this, but few replies. Is this problem trivial, or difficult? The link provided some insight, but are there not hundreds of recipes for this purpose? Thanks!
Posted: Fri Jul 09, 2010 7:53 pm
by tom jupille
I suspect that HPLC is not used very much for analysis of Fe. If you need the total Fe concentration, then something like ICP or AA would probably be a much more common solution. HPLC would come in to play primarily where you need speciation.
My guess is that you should be able to come up with a "cookbook" procedure from one of the ion chromatography vendors (e.g., Dionex or Metrohm).