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LCFA exctraction procedure

http://www.chromforum.org/viewtopic.php?t=13311

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LCFA exctraction procedure

Posted: Fri Jul 02, 2010 6:51 pm
by sribala
I want procedure for exctraction Long Chain Fatty Acids from anaerobically digested sludge samples. Please provide me the details urgently

Bala

Posted: Sat Jul 03, 2010 1:51 am
by mahdiebrahimi
You can follow Folch et al., (1957) for extraction of fat. form more information read this article : Folch, J., M. Lees and G.H. Sloan-Stanley. (1957). A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry. 226:497 – 509.

you can see this article too: http://www.informaworld.com/smpp/sectio ... =713240928

It is better to freeze-dry your samples.
Good Luck
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