Chromatography Forum

Hello,

I wonder if anyone could shed some light on an issue i am having with Chemstation and an Agilent HPLC1200

It has been working fine for the past few weeks, however, it has now started - intermittantly to stop mid sequence and say "Waiting for ready on remote line" - the VWD symbol goes yellow and it then stays there and will not go back green.

Sometimes however, it is the pump that goes yellow and displays the same message.

Maybe a coincidence but the peaks i have been obtaining since these error messages have been appearing have been negative too. I dont know if it is linked or not.

Has anyone experienced this and can point me in the right direction.

Many thanks

Craig

It sounds like you have a non-agilent component in your stack? If so then it is probably intermittantly having some sort of problem and telling the stack that it is not ready to continue.

The negative peaks are usually some species which has a higher molar absorptivity at the reference wavelength than the sample wavelength. If you have a DAD then check the spectrum of the negative component and choose an appropriate reference. Or just use the negative peaks.

Hi,

I am not aware of any non agilent parts as it was purchased and installed brand new.

In terms of the negative peaks it was working fine getting peaks with correct retention times then it started doing the negative peak at the same time as the error messages started. I did assume it was just coincidence.

Thanks for the reply

I have the same problem: on friday I changed the cooler autosampler thermostate because it was wrong. After that, I can't run any sequence, because after one or two samples, it stops and appear the message: "Waiting for ready on remote line".
Any idea?

Sorted, one of the can port leads went down, was working fine, but then became intermittant sometimes sending sometimes not sending info, change of leads and all working good

I've just call Agilent and they explained me that pump and injector must be connected only by CAN, not with REMOTE. I don't understand why I had two conections.
Luckily, now it works!!

Hello
Could be an electrical discharge in an electrical outlet.
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