Advertisement

Increasing injection volume with agilent 1100 series

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

3 posts Page 1 of 1
can anyone tell me how to increase injection volume on an agilent 1100 series autosampler. its max is 100uL.

i have a 400uL loop. is this what i need? if so where to put it?

if you could tell me where to find the info that will work too.

thanks
MestizoJoe
Analytical Chemist and Adventurer
Venture Industries
Spider-Skull Island

Check out this appnote for details on how to extend your injection capacity up to 1800 uL:

http://www.chem.agilent.com/Library/app ... 4622EN.pdf

"Large-volume injections of 1800 μL were made possible by installing the 900-μL injection upgrade kit (Agilent p/n G1363A) that includes a 900-μL analytical head, 900-μL stainless steel loop extension (Agilent p/n G1313-87303), 900-μL needle (Agilent p/n G1313-87202), and 1,400-μL extended
seat loop (Agilent p/n G13G13-87308) installed between the seat capillary fitting and injection port 5 of the analytical head."

3 posts Page 1 of 1

Who is online

In total there are 33 users online :: 2 registered, 0 hidden and 31 guests (based on users active over the past 5 minutes)
Most users ever online was 4374 on Fri Oct 03, 2025 12:41 am

Users browsing this forum: Amazon [Bot], Semrush [Bot] and 31 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry