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retinyl propionate

http://www.chromforum.org/viewtopic.php?t=1314

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retinyl propionate

Posted: Mon Jan 17, 2005 12:03 pm
by bpl
Hello to everybody

I have to do some HPLC work on compounds with retinyl propionate
Do you know any good conditions for it??
Can I use the same conditions as I do for retinol??
Thanks

Dear bpl

Posted: Tue Jan 18, 2005 3:07 pm
by Carlos Teixeira
1 - Yes, people use the same conditions for Retinol and Retinyl propionate analysis by HPLC: the polarities of both are different but the molecules are very similar.

2 - It is complex to suggest one method for you without to know how is your sample (or the matrix) but you can start with C-18 column (RP-HPLC) or Silica colunm (Normal Phase HPLC); 325 nm (the maximun absorption of Retinol); and flow at 0.6 to 1.0 mL/min. Use MeOH:Water (C-18 colunm) or Isopropanol:Heptane (Silica colunm).

Good elutions!!

Carlos Teixeira
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