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Method for compound with -COOH and -OH

http://www.chromforum.org/viewtopic.php?t=1305

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Method for compound with -COOH and -OH

Posted: Fri Jan 14, 2005 5:25 am
by Lucy
If one compound has both -COOH and -OH functional group, under acid condition it will have lactonization, what HPLC method is preferred? :oops:

Posted: Fri Jan 14, 2005 9:58 am
by HW Mueller
If your compound only has these two fuctional groups and if it indeed forms the lactone easily, and if the lactone is thermodynamically favored, and if you don´t need the ratio of the lactone to acid, you could convert it to the lactone and try a HPLC method without a buffer.
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