Hi All,
I had a problem with seperating acetylene and oxaloacetic acid with HPLC method.
Some one could help????

to clarify this problem,
the substrates I am going to seperate have identical structure and acidity (i guess).

I have tried ion exchange column, ion exclusion column, can't work this out.
Therefore , I would really need some help for separating them. Like which kind of column I would worth to try.
Thank you very much in advance.