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Hi All,
I had a problem with seperating acetylene and oxaloacetic acid with HPLC method.
Some one could help????
to clarify this problem,
the substrates I am going to seperate have identical structure and acidity (i guess).
I have tried ion exchange column, ion exclusion column, can't work this out.
Therefore , I would really need some help for separating them. Like which kind of column I would worth to try.
Thank you very much in advance.
Detection was done by a UV-Control detector at 210nm.What is your detection technique? My main concern is monitoring acetylene.
In terms of retention and separation your solutions is mixed-mode chromatography. Acetylene will retain by weak reversed-phase interaction and oxaloacetic acid will retain based on anion-exchange mechanism.
Here are few applications for neutral and acidic compounds:
http://www.sielc.com/application_165.html (vitamin C is acidic and Vitamin B2 is hydrophobic)
http://www.sielc.com/application_163.html
http://www.sielc.com/application_136.html
Contact me if you have questions.
You can also consider doing GC.