Too many peaks
Posted: Tue May 18, 2010 5:05 pm
I'm currently working on a project in which I have to first derivatize norepinephrine, and then identify its peak via fluorescence. When I inject derivatized samples of NE, I see a consistent pattern of peaks. When I inject a control sample (which contains all but the NE itself), I see no peaks. The derivatizing agents I'm using, benzylamine and DPE, should not appear under the EX and EM wavelengths of 345 and 480, respectively. However, I am seeing multiple peaks when NE is present. If NE is appearing as it should, I should only see a difference of a single peak between the control sample and NE sample. However, I consistently lose all peaks when injection the control.
1. Is it possible that NE itself is producing multiple peaks?
2. Any suggestions as to how I can identify the NE peak?
1. Is it possible that NE itself is producing multiple peaks?
2. Any suggestions as to how I can identify the NE peak?