Mineral oil in active pharmaceutical ingredients

[Fr]

I'm going to try do quantitate mineral oils in active pharmaceutical ingredients using GC/FID (6890 agilent), knowing I can use either Purged packed injector or Split/splitless or headspace (I don't think the last one can be used but..). Does anyone know something about that??

[dcobice]

Hi, well if you try to cuantitate mineral oil in these matrix I hope that using a DB-1 Or DB-5 stationary phase with FID detection is promise.The sample preparation ist depends on the solubility of the active ingredient, such as CH2Cl2 , CS2 , I-Octane or hexane are well for the extraction procces .Then you perform the chromatographic run using the last temperature about 380 degrees and Inlet temperature about 250-300 its depend the boiling point of these minerals oil. I understand that the chemistry composition of the mineral oil is round C20-C40.If you can try to use fiber glass in the inlet.Do you kown about chrom-profile for hidrocarbons, Its like a not resolved chromatogram.I hope these Help

Regards

[Fr]

Thanks for all that. Actually we're using a column from Varian (Varian CP7491,WCOT Coating Select mineral Oil : 0,10 µm, 15 meters, 0,32 mm) which is supposed to be for oils and we're closed from the things you told (solubilisation of our API in DMF and extraction with heptane). We just have to inject to see wether it works. Thanks again