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Cyclam and HPLC column

http://www.chromforum.org/viewtopic.php?t=1294

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Cyclam and HPLC column

Posted: Wed Jan 12, 2005 3:21 pm
by albatran
Hello,

I have an HPLC method. One of the method requirements is treatment of the column using Cyclam.
I do this, but I don’t now the aim. Any one can help?

Albatran

metal ion capture

Posted: Wed Jan 12, 2005 7:10 pm
by jss
Cyclam is a macromolecule ligand that forms a complex with metal ions at high pH (7< ).
If you use this compound at lower pH. you probably don't have any benefit.
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