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A gripe about GE superdex columns

http://www.chromforum.org/viewtopic.php?t=12937

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A gripe about GE superdex columns

Posted: Mon May 10, 2010 3:22 pm
by chromeleon
HiLoad 26/60
Superdex 200

this is for gel filtration. costs a fortune, and a right pain connecting to a hplc.
normal columns have an inlet and the tubing can be screwed into it, simple! not so for this

[/vent]

Posted: Mon May 24, 2010 4:19 am
by pelle-plutt
Of course these columns are a pain to connect to HPLC systems. They are designed for low pressure LC systems with a max pressure of 1.5 MPa (~215 psi).
As for the cost, I recently purchased a Sephadex 200 10/300 GL and a Phenomenex BioSep SEC S-3000. The difference in price was about 15%. I wouldn't describe this as hugely different.
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