Selected Ion Monitoring (SIM) mode
Posted: Sat Oct 25, 2025 12:42 pm
Hi there,
Sorry for asking this very basic question. I want to determine the molecular weight of a peptide (20 AAs), using Agilent LC/MS. Can I use SIM mode to determine the exact MW of my peptide. Sometimes, I am not able to get exact mass using scan mode, but with SIM mode, it mostly gives the correct mass. Is SIM mode acceptable for publication.
Thank you all for your response.
Sorry for asking this very basic question. I want to determine the molecular weight of a peptide (20 AAs), using Agilent LC/MS. Can I use SIM mode to determine the exact MW of my peptide. Sometimes, I am not able to get exact mass using scan mode, but with SIM mode, it mostly gives the correct mass. Is SIM mode acceptable for publication.
Thank you all for your response.