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HPLC detection of P-nitrobenzyl alcohol
Posted: Wed May 05, 2010 8:53 am
by S.M.PANDYA
Dear Experts
I need to know whether P-Nitrobenzyl alcohol is possible to get detected by HPLC ? If yes , can anybody suggest method ?
Thanks in advance
Posted: Wed May 05, 2010 10:13 am
by Consumer Products Guy
p-Nitrobenzyl alcohol sounds like a decent UV-absorber to me.....
You didn't state what matrix, whether you're trying to detect 10% or 10ppm, etc. That makes your question way too vague, and I'm being courteous here.
Posted: Wed May 05, 2010 11:45 am
by chromatographer1
You should do a literature search and determine the best wavelength for UV absorbance of your analyte.
Almost any analyte can be detected by refractive index and aromatic rings are strong absorbers of UV light and are easily detected by a standard UV detector.
As correctly and previously noted, it is the level of analyte that is the determining factor.
best wishes,
Rodney George
consultant