Chromatography Forum

Hello everyone
I am running a GC analysis for Fatty acid methyl esters.
We have these two reference standards (GLC-68 and GLC-85) from Nu-Chekprep Inc. for which I was not able to find the chromatograms from the company.
Can anyone provide the original chromatograms for these two standards so that I can compare with my results.

Any input will be greatly appreciated.
Regards
Sankella

You will probably have to develop your own "original" chromatograms since elution of the fatty acid methyl esters depends on your column, flow rate, oven temperature, etc. There are many fatty acid methyl ester example chromatograms on different types of columns in the GC supply catalogues and web sites. Find one that matches the column you are using and pick out the peaks that are in your Nu-Check samples.

Agree with skunked_once.

Likely Nu-Chek can provide copies from chromatograms run under THEIR conditions.

I assume you want the chromatogram for peak identity, in which case contact Nu-Check Prep and ask for a copy. Note that you will have to specify the suffix letter on the 68 ( A - F ), and any product codes.
http://www.nu-chekprep.com/

I doubt you'll find an easy way to identify peak in such complex mixtures ( 20 and 30 component fatty acids). Some polyunsaturated individual fatty acids will polymerise on standing ( which is why I prefer to use freshly-prepared natural fish oil mixtures, rather than synthetic - unless mandated by AOCS etc ), so if you vials have been opened they may not match the profile. It may be better to purchase fresh batches, but if not, regard all values for Cx:3 to Cx:6 fatty acid methyl esters with suspicion.

As always, the first port of call should be the manufacturer, ask nicely, and give them all details on the container labels. I'll be surprised if they don't provide relevant chromatograms.